Table 1.
The deaminase activity and mC selectivity factor of A3H variants
| Deaminase Activity | hap I | Splicing variant 182 (SV182) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| SV 200 | SV 183 | SV 182 | SV 154 | hap I | hap II | hap III | hap IV | hap V | hap VI | hap VII | |
| Act. on Ca (nM product /μM A3H/hr) | 870 ±92 | 1183 ±42 | 1283 ±102 | 0 | 1424 ±105 | 21809 ±429 | 0 | 0 | 36986 ±3046 | 0 | 24657 ±1713 |
| Act. on mCa (nM product /μM A3H/hr) | 119±6 | 158±5 | 160±5 | 0 | 215 ±17 | 18559 ±1121 | 0 | 0 | 4807 ±76 | 0 | 7050 ±336 |
| mC selectivity factorb | 137 | 13.4 | 12.5 | 0 | 15.1 | 85.1 | 0 | 0 | 13.1 | 0 | 28 6 |
Note:
a
Deaminase activity (nM product /μM enzyme/hr) for the C and mC deamination was calculated from the initial linear range of dose response assays for each protein constructs using the 30 nt ssDNA substrate containing motif TCA. SD was estimated from data collected in three independent experiments.
b
The mC selectivity factor was calculated [mC/C activity × 100], where the deaminase activity for the corresponding C and mC for each A3H variant in this table was used for the calculation.