Fig. 3.
Detection of GC in E. sonchifolia leaf tissue infected with TSWV SLIs. Samples were analyzed by SDS/PAGE and Western blot. GC was detected in the plant leaf tissue infected with thrips transmissible SLIs (37 and 41; lanes 2 and 3) and nontransmissible SLI (81; lane 7), which form TSWV virions in E. sonchifolia leaf tissue, whereas GC was not detected in E. sonchifolia leaf tissue infected with nontransmissible SLIs (2, 61, and 80; lanes 1, 5, and 6), which have nonsense or frameshift mutations or both in the GN/GC ORF. GC was not detected in E. sonchifolia leaf tissue infected with SLI 60 which has mutations of A1202G (K401R) and G2294U (G765V) in GN/GC ORF (lane 4). Healthy leaf tissues and leaf tissues infected with the TSWV-RG2P24 were used as negative and positive controls, respectively (lanes 8 and 9).