Table 1. Thrips transmissibility of TSWV reassortants generated from transmissible (RG2; LRMRSR) and nontransmissible (D; LDMDSD) isolates.
| Reassortant | Transmission, infected/inoculated* | Genotype† |
|---|---|---|
| LDMDSD | 0/10; 0/10; 0/10 | NA |
| LRMRSR | 6/9; 3/10; 2/10 | LRMRSR |
| LRMRSD | 4/10; 2/10; 2/10 | LRMRSD |
| LRMDSD | 0/10; 0/10; 0/10 | NA |
| LRMDSR | 0/10; 0/10; 0/10 | NA |
| LDMDSR | 0/10; 0/10; 0/10 | NA |
| LDMRSR | 4/10; 3/10; 3/10 | LDMRSR |
| LDMRSD | 4/10; 2/10; 2/10 | LDMRSD |
*
Thrips transmissibility was confirmed by symptom development and RT-PCR after inoculation by thrips. Individual experiments are separated by semicolon. The numerator and the denominator represent the number of infected and inoculated plants, respectively. Each plant was exposed to 10 potentially viruliferous thrips.
†
Genotype of each symptomatic leaf tissue after thrips transmission was determined by RT-PCR and RFLP.