Table 2. Mutation analysis of the M RNA of thrips transmissible and nontransmissible SLIs generated from TSWV-RG2P24.
| Mutation
|
||||||
|---|---|---|---|---|---|---|
| Thrips transmissibility*
|
NSm, IGR, 5′ and 3′ NTR
|
GN (1-1452)†
|
GC (1453-3408)†
|
|||
| SLI | S | NS | S | NS | ||
| 2 | 0/30; 0/30; 0/30 | — | — | A deletion at 310, C1375A (P459T)‡ | — | — |
| 11 | 0/30; 0/30; 0/30 | — | — | A deletion at 270, C1375A (P459T) | — | — |
| 28 | 0/30; 0/30; 0/30 | — | — | A923G (K308R), C1375A (P459T) | — | — |
| 37 | 9/30; 8/30; 8/30 | — | — | — | U1683C | — |
| 41 | 7/30; 6/30; 7/30 | — | — | — | U1683C | — |
| 43 | 0/30; 0/30; 0/30 | — | — | A776G (H259R), C1375A (P459T) | — | — |
| 80 | 0/30; 0/30; 0/30 | — | — | U144A (stop§) C1054U (L352F) | U1683C | Addition of UUU at 1977 G1975U (V659L) |
| 81 | 0/30; 0/30; 0/30 | — | — | C1375A (P459T) | — | — |
IGR, intergenic region; NTR, nontranslated region; S, synonymous; NS, nonsynonymous; -, no mutation found.
*
Thrips transmissibility was confirmed by symptom development and RT-PCR after inoculation by thrips. Individual experiments are separated by semicolon. The numerator and the denominator represent the number of infected and inoculated plants, respectively. Each plant was exposed to 10 potentially viruliferous thrips.
†
The GN/GC annotation was based on refs. 18 and 47. Numbers designate nucleotide number from first nucleotide in ORF.
‡
C1375A (P459T) means that the nucleotide at 1375 was substituted from C to A, which changes amino acid 459 from Pro (P) to Thr (T).
§
Stop means mutation which converts codon to translation termination codon.