Figure 1.

TGF-β induces efficient G1 cell cycle suppression in MM.1S cells. (A) The MM.1S cell lines were either left untreated or treated with 5 ng/ml TGF-β for the indicated times and assessed for cell viability by MTS. (B) Cells were treated as aforementioned, and the cell count was determined at the indicated time points. Results are expressed as the mean ± standard deviation of two independent experiments, with each performed in triplicate. (C) MM.1S cells were seeded at a density of 3×105 cells/ml overnight, stained with propidium iodide, and analyzed by flow cytometry. Bar graphs show the percentage of MM.1S cells in each cell cycle phase for three independent experiments. TGF-β, transforming growth factor-β. *P<0.05.