Figure 7.

Effect of ERK1/2 and JNK inhibitors on the level of P-ERK, P-JNK and stathmin. (A) A western blot analysis was used to detect the expression of ERK in MG63/dox cells treated with PD098095. (B) MG63/dox cells were treated with 2 µM As₂O₃ and 200 ng/ml ADM for 48 h. A western blot analysis was used to detect the expression of JNK in the cells treated with SP600125. The expression of stathmin in the MG63/dox cells with (C) PD098095 and (D) As₂O₃, ADM and SP600125 treatment was also detected by a western blot analysis. β-actin was used as a loading control. *P<0.05 compared with cells untreated with inhibitor. ERK1/2, mitogen-activated protein kinase 3/1; JNK, mitogen-activated protein kinase 8; P-ERK, phosphorylated-mitogen-activated protein kinase 3/1; P-JNK, phosphorylated-mitogen-activated protein kinase 8; MG63/dox, MG63/doxorubicin-resistant; As₂O₃, arsenic trioxide; ADM, doxorubicin.