Fig. 6.

Plx1 is required for the destruction of Emi2. (A) Depletion of Plx1 from egg extract. CSF extract was either mock-depleted with IgG beads or depleted with Plx1 antibodies for two rounds of depletion. Depleted extract or beads were immunoblotted for Plx1 protein. (B) Depletion of Plx1 prevents CSF release. CSF extract depleted as above was assayed for cyclin B2 destruction and H1 kinase inactivation with or without calcium addition. (C) Depletion of Plx1 stabilizes Emi2 in calcium-stimulated CSF extract. CSF extract depleted as above was incubated with IVT Emi2 with or without calcium addition, and samples were collected for autoradiography at the indicated times. (D) Plk1 stimulates the binding of hEmi2 to βTrCP.