Figure 2.

Silencing of CERS4 expression by lentivirus-mediated RNAi. Representative images of (A) HepG2 and (B) Huh7 cells infected by recombinant lentivirus. Reverse transcription-quantitative polymerase chain reaction assay to detect the knockdown efficiency of CERS4 in (C) HepG2 and (D) Huh7 cells (n=3). The knockdown efficiency of CERS4 determined by western blot analysis in (E) HepG2 and (F) Huh7 cells. The values are presented as the mean ± standard error of the mean. ***P<0.001. CERS4, ceramide synthase-4.