Figure 6.

Effects of ceramide synthase-4 knockdown on the NF-κB signaling pathway. The mRNA levels of (A) p53, (B) Gsk-3β, (C) β-catenin1, (D) Ikbkg and (E) Tank detected reverse transcription-quantitative polymerase chain reaction (n=3). (F) The protein levels of NF-κB in the cytoplasm and cell nucleus detected by western blot analysis. The values are presented as the mean ± standard error of mean.***P<0.001. Gsk-3β, glycogen synthase kinase-3β; Ikbkg, inhibitor of κ-light polypeptide gene enhancer in B-cells kinase γ; NF-κB, nuclear factor-κB.