(A) Summary of RT-PCR and MS-PCR results. 47 samples with PCDH8 methylation all showed decreased PCDH8 expression. The overlapping ratio was 100%. (B) Methylation statuses of PCDH8 from 4 representative patients with prostate cancer. Cases 22 and 24 exhibited PCDH8 promoter methylation. PCDH8, protocadherin 8; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MS-PCR, methylation-specific polymerase chain reaction; M, methylated; U, unmethylated.