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. 2017 Jun 8;14(2):1536–1542. doi: 10.3892/ol.2017.6350

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Copyright: © Cai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — CMTM5 induces cell cycle arrest and apoptosis. (A) The histograms show the percentages of cells in each cell cycle phase, as determined by the gating of the flow cytometer. (B) ACHN cells infected with NC and CMTM5-OE were labeled with fluoresce in isothiocyanate-Annexin V/PI, and apoptosis was assessed by flow cytometry. (C) Percentages of cells in each cell cycle phase. (D) Apoptosis rate of cells infected with NC and CMTM5-OE after 72 h. ACHN NC refers to ACHN cells infected with the lenti-EGFP, while CMTM5 OE refers to ACHN cells infected with CMTM5 overexpressing lentivirus. CMTM5, CKLF-like MARVEL transmembrane domain-containing 5; CMTM5-OE, CMTM5 over-expression lentivirus; NC, negative control; OE, overexpression; PI, propidium iodide.