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Esculetin induces the phosphorylation of β-catenin. SMMC-7721 cells were treated with 100 or 300 µmol/l esculetin or dimethyl sulfoxide vehicle for 24 h. Total protein was extracted for western blot analysis of β-catenin and p-β-catenin (Ser33/Ser37/Thr41), with β-actin as a loading control. p-β-catenin, phosphorylated β-catenin.
