Figure 6.

Celastrol induces cell apoptosis by the death receptor and mitochondrial pathways. Cells were treated with celastrol, at doses of 1, 2 or 4 µM, for 24 h. (A) The mitochondrial membrane potential was analyzed with flow cytometry. (B) Western blot analysis detected the change in the expression level of Fas, FADD, TRADD, TNF-R1 and DR5. (C) The western blot was quantified, with detected protein levels normalized to β-actin using a densitometer. *P<0.05 vs. 0 µM control. FADD, Fas associated via death domain; TRADD, TNFRSF1A associated via death domain; TNF-R1, TNF receptor superfamily member 1A; DR5, TNF receptor superfamily member 10B.