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. 2015 Dec 30;66(4):692–704. doi: 10.1136/gutjnl-2015-310016

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Colorectal cancers (CRC)-Th17 mediate protumorigenic effects in an interleukin (IL)-17-dependent manner. Endothelial cells (EC), tumour-associated stromal cells (TASC) and CRC cell lines (LS180, COLO205) were conditioned for 24 h with CRC-Th17 supernatants untreated (Th17 SN) or pretreated with anti-IL-17 neutralising antibodies (Th17+ anti-IL-17). Vascular endothelial growth factor (VEGF) (A) or IL-6 (B) release was measured in culture supernatants by ELISA. Statistical significance was analysed by one-way analysis of variance. Data refer to experimental triplicates from two independent experiments performed with supernatants from two different clones. Ctr, healthy colonic tissue.