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. 2015 Dec 30;66(4):692–704. doi: 10.1136/gutjnl-2015-310016

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Colorectal cancers (CRC)-infiltrating Th17 favour recruitment and activation of neutrophils. (A) Migration of neutrophils, purified from blood of healthy donors, towards control medium (Ctr), supernatants of Th17 clones expanded from CRC-infiltrating cells (Th17 SN) or Th17 supernatants pre-treated with anti-interleukin (IL)-17 (Th17 SN+ anti-IL-17), anti-IL-8 (Th17 SN+ anti-IL-8), or anti-granulocyte-macrophage colony stimulating factor (anti-GM-CSF antibodies) (Th17 SN+ anti-GM-CSF), was evaluated after 90 min incubation by flow cytometry. Data refer to experimental triplicates from three independent experiments performed with supernatants from three different clones. Means±SD are depicted. Statistical significance was assessed by one-way analysis of variance (ANOVA). (B) Myeloperoxidase (MPO) release by neutrophils exposed to control medium (Ctr), Th17 supernatants (Th17 SN) or Th17 supernatants pretreated with anti-IL-17, IL-8 or GM-CSF antibodies was assessed after 4 h incubation by ELISA. Means±SD are depicted. Data refer to experimental triplicates from three independent experiments performed with supernatants from three different clones. Statistical significance was assessed by one-way ANOVA.