Fig. 3. Confocal microscopy of (a) azido-E. coli or (b) GFP-E. coli after phagocytosis. BM-DCs were pulsed with either azido-E. coli or GFP-expressing E. coli (45 min pulse). Cells were fixed after a 2 h chase and stained with DAPI (blue), anti-actin (red) and, in case of azido-E. coli, AlexaFluor-488 alkyne (green = either GFP or AlexaFluor-488). (i) DAPI/488 nm overlay; (ii) DAPI only; (iii) all fluorescent channels overlay. Yellow arrows indicate a 488-single positive focus, white arrows a DAPI/488 nm double positive focus.
