Fig. 5. CLEM imaging of bioorthogonal tagged sialylated glycans in Jurkat cells. Green: AlexaFluor-488 alkyne; blue: DAPI. DAPI staining was used for correlation purposes. (a) Confocal microscopy images; (b) EM image; (c) CLEM image obtained from overlay LM and EM pictures; (e) CLEM detail from (c) of intracellular region; CLEM detail from (c) of plasma membrane. Scale bar 2 μm.
