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. 2017 Jul 18;10:3545–3556. doi: 10.2147/OTT.S134162

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© 2017 Shen et al. This work is published and licensed by Dove Medical Press Limited

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Epitope mapping by peptide array-based immunological screening.

Notes: (A) Nine groups of pooled serum from NSCLC patients (n=72) and two groups from healthy controls (n=16) and commercial DKK1 monoclonal antibody were used to probe DKK1 microarrays, respectively. The spots A1 and D22 are the positive controls. (B) Plot of DKK1 peptides was in response to NSCLC and healthy serum samples. Samples with intensity value greater than half of the positive controls were considered as seropositive reaction. (C) Four peptides, shown in black box, were selected as highly responsive peptides.

Abbreviations: DKK1, dickkopf-1; NSCLC, non-small cell lung cancer.