Figure 1.

Expression of mature miR-34a in colorectal cancer cells and tissues. (A) Reverse transcription-quantitative polymerase chain reaction analysis of miR-34a expression in the potentially invasive colon cancer cell lines HT29, SW480 and SW620, and the normal colonic epithelial cell line NCM460. The data are expressed as the mean ± standard deviation (n=3). *P<0.05 and **P<0.01 vs. NCM460. (B) Colorectal cancer tissue microarray composed of normal colon tissue and colon adenocarcinoma tissue from patients was analyzed using in situ hybridization. Representative images of miR-34a expression (left panel: Original magnification, ×100; scale bar, 100 µm; right panel: Original magnification, ×200; scale bar, 50 µm) (C) Representative images of miR-34a expression in well-differentiated (high/moderate differentiation) colorectal cancer, poorly differentiated (low differentiation) colorectal cancer, non-metastatic tissues and metastasis tissues. Original magnification, ×200; scale bar, 50 µm. (D) Kaplan-Meier estimator survival curves for patients according to the expression levels of miR-34a in tumors. miR, microRNA.