Figure 3.

miR-34a inhibits tumor cell migration and invasion by targeting Notch1 and Jagged1. (A) SW480 cells were transfected with miR-34a (or mimic NC) followed by Notch1 or Jagged1 transfection. Cells were subjected to the wound closure assay in a time-dependent manner. Images captured at 0 and 48 h are provided. Representative images of the migrated stained cells are also provided (left). Cells in 3 randomly selected areas were counted and statistical analyses were performed. The data are expressed as the mean ± SD (n=3) (right). *P<0.05, **P<0.01 and ***P<0.001 vs. miR-34a mimic. (B) Transwell migration assays examining the effects of miR-34a on cell invasion ability. SW480 cells were transfected with miR-34a (or mimic NC) followed by Notch1 or Jagged1 transfection. The Transwell migration assay was performed 24 and 36 h later. Representative images of the migrated stained cells are provided (left). SW480 cells in 5 randomly selected areas were counted and statistical analyses were performed (right). The data are expressed as the mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-34a mimic. (C) Regulation of the expression of E-cadherin, vimentin, fibronectin, Slug, Snail and ZEB1 by miR-34a. SW480 cells were transfected with an miR-34a mimic or mimic NC for 48 h. The expression of E-cadherin, vimentin, fibronectin, Slug, Snail and ZEB1 was analyzed using the reverse transcription-quantitative polymerase chain reaction. Values were normalized to GAPDH as an internal control. The data are expressed as the mean ± SD (n=3). *P<0.05 vs. mimic NC. (D) SW480 cells were transfected with miR-34a (or mimic NC) followed by Notch1 or Jagged1 transfection. The protein expression of vimentin and fibronectin was assayed by western blot analysis. miR, microRNA; SD, standard deviation; E-cadherin, epithelial cadherin; Slug, zinc finger protein SNAI2; Snai1, zinc finger protein SNAI1; ZEB1, zinc finger E-box-binding homeobox 1; NC, negative control.