Table I.
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|
Pre-microRNA-34a | UGGCAGUGUCUUAGCUGGUUGU | AACCAGCUAAGACACUGCCAUU |
Notch1 | AGCCTCAACATCCCCTACAA | CCACGAAGAACAGAAGCACA |
Jagged1 | ACGGGAAGTGCAAGAGTCAG | GTTTCACAGTAGGCCCCCTC |
Epithelial cadherin | TTCTGGAAGGAATGGAGGAGTC | ACCTGGAATTGGGCAAATGTG |
Vimentin | AGATGGCCCTTGACATTGAG | TGGAAGAGGCAGAGAAATCC |
Fibronectin | GGTGACACTTATGAGCGTCCTAAA | AACATGTAACCACCAGTCTCATGTG |
ZEB1 | GCACAACCAAGTGCAGAAGA | GCCTGGTTCAGGAGAAGATG |
Snail | GCTGCAGGACTCTAATCCAGAGTT | GACAGAGTCCCAGATGAGCATTG |
Slug | AGATGCATATTCGGACCCAC | CCTCATGTTTGTGCAGGAGA |
GAPDH | AACGGATTTGGTCGTATTGG | TTGATTTTGGAGGGATCTCG |
Slug, zinc finger protein SNAI2; Snai1, zinc finger protein SNAI1; ZEB1, zinc finger E-box-binding homeobox 1.