Table II.

Primers and oligonucleotides.

	Forward primer (5′-3′)	Reverse primer (5′-3′)
3′UTR of wild 1 Notch1	CGCGTTTTATTTATGTACTTTTATTT	AGCTTTACATATAAATAAAAAGG
	TACACAGAAACACTGCCTTTTTATT	CAGTGTTTCTGTGTAAAATAAA
	TATATGTAA	AGTACATAAATAAAA
3′UTR of mutant1 Notch1	CGCGTTTTATTTATGTACTTTT	AGCTTTACATATAAATAAAAAA
	ATTTTACACAGAAAAAAATTTTTTT	AATTTTTTTCTGTGTAAAATAAAAG
	TATTTATATGTAA	TACATAAATAAAA
3′UTR of wild2 Notch1	CGCGTTACCCTTTTCTGGGGAA	AGCTTGCTGGGGCCGCCACCG
	AGACACTGCCTGGGCTGACCCCGGT	GGGTCAGCCCAGGCAGTGTCTTTC
	GGCGGCCCCAGCA	CCCAGAAAAGGGTAA
3′UTR of mutant12 Notch1	CGCGTTACCCTTTTCTGGGGAAA	AGCTTGCTGGGGCCGCCACCG
	GAAAAATTTTGGGCTGACCCCGGT	GGGTCAGCCCAAAATTTTTCTTTCC
	GGCGGCCCCAGCA	CCAGAAAAGGGTAA
3′UTR of wild 1 Jagged1	CGCGTACCGCGGGCACTGCCGCCG	AGCTTTTAAAGAACTACAAGC
	CTAGGTAGAGTCTGAGGGCTTGTAG	CCTCAGACTCTACCTAGCG
	TTCTTTAAA	GCGGCAGTGCCCGCGGTA
3′UTR of mutant1 Jagged1	CGCGTACCGCGGGAAAATTTGC	AGCTTTTAAAGAACTACAAGC
	CGCTAGGTAGAGTCTGAGGGCTTGT	CCTCAGACTCTACCTAGCGGC
	AGTTCTTTAAA	AAATTTTCCCGCGGTA
3′UTR of wild2 Jagged1	CGCGTTTTAGATTTGCCATAGAGTA	AGCTTCTTTGATTTCCTCACTTAA
	CACTGCCTGCCTTAAGTGAGGAA	GGCAGGCAGTGTACTCTATG
	ATCAAAGA	GCAAATCTAAAA
3′UTR of mutant2 Jagged1	CGCGTTTTAGATTTGCCATAGAGTA	AGCTTCTTTGATTTCCTCACTTAA
	AAAATTTTGCCTTAAGTGAGG	GGCAAAATTTTTACTCTATG
	AAATCAAAGA	GCAAATCTAAAA

The underlined sections of the sequences are mutation sites. UTR, untranslated region.