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Analyses of Wnt2, Wnt5a, Axin2 and Wnt target gene (survivin, c-Myc, cyclin D1 and VEGF) expression in Colo16 cells without and with 100 µM resveratrol treatment for 48 h by RT-PCR and western blotting. β-actin was used as a quantitative control. Gray density analysis was conducted on the Wnt2, Wnt5a, Axin2 and Wnt target gene data. N, normal culture; R, 100 µM resveratrol treatment; VEGF, vascular endothelial growth factor; RT-PCR, reverse transcription-polymerase chain reaction.
