Representative sequencing electropherograms of the potential mutation site in ERK2 p.E322, ERK1 p.E339, KRAS (p.G13 and p.Q61), NRAS (p.G12, p.G13 and p.Q61), HRAS (p.G12, p.G13 and p.Q61) and BRAF (p.V600E); the arrows refer to the wild type sequence with potential hotspot mutations. ERK, mitogen-activated protein kinase; KRAS, Kirsten rat sarcoma viral oncogene homolog; NRAS, neuroblastoma RAS; HRAS, Harvey RAS; BRAF, B-Raf proto-oncogene serine/threonine kinase.