Figure 5.

miR-452 directly targets RAB11A in breast cancer cells. The relative RAB11A (A) mRNA and (B) protein expression levels were significantly reduced in the miR-452 mimic-transfected breast cancer cells, compared with miR-NC-transfected cells. (C) A luciferase reporter assay was performed on HEK293T cells co-transfected with an miR-452 mimic or miR-NC, and PmirGLO-RAB11A-3′-UTR-WT or PmirGLO-RAB11A-3′-UTR-Mut, revealing that miR-452 decreased activity only in cells transfected with the WT RAB11A 3′-UTR. Data are presented as the mean ± standard deviation. *P<0.05 vs. corresponding miR-NC group. miR-452, microRNA-452; miR-NC, microRNA negative control; 3′-UTR, 3′-untranslated region; WT, wild type; Mut, mutant.