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. 2017 Jun 19;14(2):2081–2088. doi: 10.3892/ol.2017.6416

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Expression of E1A in the 5,637 cell line following treatment with MMC or HCPT. A total of 5,637 cells was infected with recombinant Ad5-UPII-E1A (10 MOI) alone or with Ad5-UPII-E1A combined with MMC or HCPT for 72 h. The results revealed that, following exposure to Ad5-UPII-E1A (10 MOI) combined with different doses of MMC and HCPT for 72 h, E1A was increased compared with Ad5-UPII-E1A alone, and is increase was dependent on the dose of MMC and HCPT. GAPDH was used as a loading control. E1A, adenoviral early region gene; MMC, mitomycin; HCPT, hydroxycamptothecin; Ad5-UPII-E1A, urothelium-specific recombinant adenovirus type 5; 10 MOI, 10 multiplicity of infection.