RPESP is expressed in certain colon cancer cell lines. (A) Expression of RPESP mRNA in human tumor cell lines. Total RNAs were isolated from A549 lung adenocarcinoma, ES2 ovarian cancer, HepG2 hepatoma, HT1080 fibrosarcoma, HT29 colon cancer, Jurkat leukemia, LNCaP prostate cancer and WM266-4 melanoma cells and RT-sqPCR analysis was performed. (B) Expression of RPESP mRNA in human colon cancer cell lines. Total RNAs were isolated from human colon cancer cell lines, HT29, COLO 205, CW-2, HCT116 and LoVo, and RT-sqPCR analysis was performed. RT-sqPCR, reverse transcription-semi-quantitative polymerase chain reaction; RPESP, RPE-spondin.