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. 2017 Jun 21;14(2):2261–2267. doi: 10.3892/ol.2017.6439

Figure 4.

Figure 4.

miR-340 negatively regulates ROCK1 by binding to the ROCK1 3′-UTR. (A) Binding sites of wild-type and mutant ROCK1 3′-UTR. (B) Luciferase reporter assay showing the inhibitory effect of miR-340 on ROCK1 3′-UTR luciferase activity (P<0.05; miR-340 mimics vs. mimic NC). (C) Reverse transcription-quantitative polymerase chain reaction showing ROCK1 mRNA level in MDA-MB-231 cells, comparing miR-340 mimics with miR-340 inhibitor. (D) Western blotting comparing ROCK1 protein level with NC. miR-340 mimics downregulated the expression of ROCK1, whereas miR-340 inhibitor upregulated ROCK1 expression. P<0.05 for miR-340 mimics vs. mimic NC; miR-340 inhibitors vs. inhibitor NC. ROCK1, Rho-associated, coiled-coil containing protein kinase 1; NC, negative control; miR, microRNA; UTR, untranslated region; mRNA, messenger RNA; hsa, Homo sapiens.