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. 2017 Jun 21;14(2):2261–2267. doi: 10.3892/ol.2017.6439

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Copyright: © Maskey et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Knockdown of ROCK1 in MDA-MB-231 cells by siRNA. (A) Downregulation of ROCK1 by siRNA was confirmed by reverse transcription-quantitative polymerase chain reaction and western blotting in MDA-MB-231 cells. (B) Growth curve comparing siRNA of ROCK1 vs. siRNA of NC. (C) Colony formation assay comparing siRNA of ROCK1 vs. siRNA of NC. (D) Migration assay comparing siRNA of ROCK1 vs. siRNA of NC. (E) Invasion assay comparing siRNA of ROCK1 vs. siRNA of NC. All these show suppressive function of siRNA of ROCK1. P<0.05; siRNA-ROCK1 vs. siRNA NC. ROCK1, Rho-associated, coiled-coil containing protein kinase 1; NC, negative control; miR, microRNA; siRNA, short interfering RNA; OD, optical density.