Figure 3.

Effect of H19 overexpression on viability, migration and expression level of endothelial-mesenchymal transition-associated proteins. (A) Expression level of H19 determined using the quantitative polymerase chain reaction 3 days after transfection with H19-expressing plasmid or control plasmid, in HCT-116 and SW-480 cells. Cells transfected with H19-expressing plasmid exhibited a markedly increased expression level of H19, compared with cells transfected with the control plasmid (vector). (B) Cells were transfected with the H19 expressing plasmid or the control plasmid for 24 h and incubated for 5 days, followed by MTT assays. The viability level of cells overexpressing H19 is markedly increased, compared with cells transfected with the control plasmid (vector). (C) Migratory capacity of cells overexpressing H19 was markedly increased compared with cells transfected with the control plasmid (vector). (D) Expression levels of E-cadherin, vimentin and Snail were determined in HCT-116 and SW-480 cells 72 h after transfection with H19 or control plasmid. All experiments were performed in triplicate and repeated 3 times. Results are presented as the mean ± standard error of the mean (n=3) (**P<0.01 vs. control). GAPDH was used as a loading control. H19, long non-coding RNA-imprinted maternally expressed transcript; E-cadherin, epithelial cadherin.