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. Author manuscript; available in PMC: 2017 Jul 27.
Published in final edited form as: Structure. 2017 Apr 4;25(4):575–577. doi: 10.1016/j.str.2017.03.013

Figure 1. CARD Disk in the Apaf-1 Apoptosome.

Figure 1

(A) Domain architectures of Apaf-1, caspase-9, and cytochrome c.

(B) Top view of the cryo-EM structure of the Apaf-1 apoptosome (PDB ID: 3JBT).

(C) Side view of the Apaf-1 holo-apoptosome showing the 4:4 Apaf-1:caspase-9 CARD disk above the heptameric wheel-like structure (PDB ID: 5JUY).

(D) A schematic of structurally captured steps in CARD disk assembly and activation of Apaf-1 holo-apoptosome.

(E) Superimposed type I, II, and III (for both Apaf-1:Apaf-1 and caspase-9:caspase-9) interactions between those in the crystal structure (PDB ID: 5WVC, Apaf-1 in slate and caspase-9 in orange) and the cryo-EM structure (PDB ID: 5JUY, Apaf-1 in cyan and caspase-9 in yellow).