Figure 2. The pH effect on deaminase activity.

The deaminase activity assay of the purified APOBECs were performed at pH 5.5, 6.5, 7.5, and 8.5. 30 nt ssDNA containing the preferred tri-nucleotide motifs for the respective APOBEC members were used. The tri-nucleotide motifs used for each protein, and the positions of the 30 nt substrates and the 16 or 17 nt products are indicated next to each gel image. The deaminase assay was performed as described in Fig. 1D.