Table 3.

Deaminase activity on C and mC of wt and mutant A3As.

Enzyme		C deaminase activity (nM prod/μM enz)	mC deaminase activity (nM prod/μM enz)	mC selectivity factor (mC/C) x 100
A3A	wt	266,000 ± 6,000	33,900 ± 400	12.7
	R1	3,180 ± 150	153 ± 3	4.8
	R2	25,500 ± 600	3,440 ± 70	13.5
	R3	322,000 ± 79,000	42,300 ± 1,100	13.1
	R1-1	352,000 ± 14,000	42,200 ± 800	12.0
	R1-2	6,400 ± 480	391 ± 19	6.1
	R1-3	9,430 ± 350	444 ± 7	4.7

Note: Deaminase activity for C and mC was calculated from the initial linear range of dose dependent product formation for each A3A variant (Fig. 4C–I). S.D. was estimated from data collected in three independent deaminase assay experiments. The mC selectivity factor was calculated as mC/C activity x 100.