Table II.
RT-qPCR cycling conditions.
| Number of cycles | Duration (sec) | Temperature (°C) | Optic detection |
|---|---|---|---|
| 1 | 600 | 95 | Off |
| 45 | 15 | 95 | Off |
| 30–40 | 60 | Off | |
| 30 | 72 | On |
RT, reverse transcriptase; qPCR, quantitative polymerase chain reaction.
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.