Table 4.
Histomorphometry of lumbar vertebrae
| WT-CTRL | WT-CKD | SOST-KO-CTRL | SOST-KO-CKD | P WT-CTRL versus SOST-KO-CTRL | P WT-CTRL versus WT-CKD | P SOST-K OCTRL versus SOST-KO-CKD | P for interaction (2-way ANOVA) | |
|---|---|---|---|---|---|---|---|---|
| BV/TV (%) | 21 ± 5 | 23 ±5 | 49 ±8 | 42 ±5.1 | <0.001 | 0.220 | 0.02 | 0.01 |
| Tb.Th (μm) | 39 ± 4 | 34 ± 4 | 63 ± 11 | 46 ± 5 | <0.001 | 0.002 | <0.001 | 0.001 |
| Tb.Sp (μm) | 158 ± 49 | 114 ± 27 | 68 ± 19 | 64 ± 13 | <0.001 | 0.006 | 0.5 | 0.02 |
| OV/BV (%) | 0.6 (0.2–1.1) | 2.3 (1.6–6.7) | 1.1 (0.1–1.4) | 1.8 (0.7–2.9) | 0.7 | <0.001 | 0.028 | 0.2 |
| OS/BS (%) | 5.7 (1.4–8.1) | 9.5 (7.0–19.1) | 7.9 9 (1.6–10.9) | 9.1 (4.6–14.2) | 0.4 | 0.004 | 0.2 | 0.2 |
| O.Th (μm) | 2.5 (2.1–3.0) | 4.8 (4.1–5.7) | 4.0 (2.7–4.6) | 4.8 (4.0–5.6) | 0.01 | <0.001 | 0.01 | 0.02 |
| Ob.N (1/100 mm) | 142 (47–319) | 95 (64–202) | 117 (73–202) | 74 (40–99) | 0.6 | 0.5 | 0.2 | 0.6 |
| Ob.S/BS (%) | 2.2 (0.7–3.5) | 1.4 (0.8–2.8) | 1.6 (0.9–3.2) | 1.0 (0.3–1.2) | 0.5 | 0.5 | 0.1 | 0.5 |
| ES/BS (%) | 2.0 (1.2–2.9) | 2.1 (1.3–5.1) | 1.6 (1.1–2.1) | 1.3 (0.6–1.8) | 0.5 | 0.3 | 0.2 | 0.1 |
| Oc.N (1/100 mm) | 52 (34–78) | 76 (51–153) | 53 (40–77) | 33 (21–45) | 0.9 | 0.08 | 0.06 | 0.01 |
| Oc.S/BS (%) | 1.7 (1.1–2.7) | 1.6 (1.2–4.4) | 1.2 (0.8–2.0) | 1.1 (0.7–1.3) | 0.6 | 0.6 | 0.1 | 0.1 |
| MAR (μm/d) | 1.0 (0.6–1.4) | 0.9 (0.7–1.6) | 1.4 (0.8–1.8) | 0.8 (0.7–1.0) | 0.2 | 0.5 | 0.1 | 0.1 |
| DLS (%) | 6.7 ±3.0 | 4.4 ±3.5 | 8.9 ±4.5 | 9.1 ± 2.7 | 0.2 | 0.08 | 0.9 | 0.02 |
| SLS (%) | 5.2 ±2.7 | 4.8 ±2.3 | 3.7 ±0.8 | 3.2 ± 1.6 | 0.08 | 0.7 | 0.4 | 0.6 |
| MS/BS (%) | 8.7 (6.4–12.2) | 5.4 (4.0–7.8) | 10.6 (6.4–15.1) | 10.3 (8.1–12.8) | 0.6 | 0.03 | 0.7 | 0.07 |
| BFR/BS (mm3/cm2/y) | 2.5 (2.0–6.1) | 2.1 (1.8–3.8) | 4.1 (3.1–6.2) | 3.6 (2.8–4.3) | 0.1 | 0.3 | 0.3 | 0.8 |
| Mlt (d) | 1.2 (0.7–2.6) | 9.2 (2.3–18.1) | 1.9 (0.7–3.2) | 6.1 (2.7–7.5) | 0.4 | <0.001 | 0.04 | 0.06 |
| Omt (d) | 3.2 ±1.3 | 5.7 ±2.6 | 3.4 ± 1.4 | 5.5 ±1.7 | 0.7 | 0.004 | 0.003 | 0.8 |
ANOVA, analysis of variance; BFR/BS, bone formation rate/bone surface; BV/TV, bone volume/total volume; CKD, chronic kidney disease; CTRL, control; DLS, double-labeled surface; ES/BS, erosion surface/bone surface; KO, knockout; MAR, mineral apposition rate; Mlt, mineralization lag time; MS/BS, mineralizing surface/bone surface; Ob.N, osteoblast number/bone length; Ob.S/BS, osteoblast surface/bone surface; Oc.N, osteoclast number/bone length; Oc.S/BS, osteoclast surface/bone surface; Omt, osteoid maturation time; OS/BS, osteoid surface/bone surface; O.Th, osteoid thickness; OV/BV, osteoid volume/bone volume; SLS, single-labeled surface; SOST, sclerostin; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness; WT, wild-type.
Data are shown as mean ±SD or median (25th to 75th percentiles).