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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Antiviral Res. 2017 Apr 23;143:162–175. doi: 10.1016/j.antiviral.2017.04.011

Figure 1. Growth kinetics of MP12 and its effect on p70 S6K signaling in H2.35 cells.

Figure 1

(A) H2.35 cells were infected with MP12 at an MOI 5.0. Cell lysates were collected 3, 9, 18 and 24 hpi and viral titers were measured by standard plaque assay (B) H2.35 cells were serum starved for 72 hours and then either mock-infected or infected with MP12 at an MOI 5.0. Cell lysates were collected at 3, 9, 18 and 24 hours post-infection and analyzed by western blot. Membranes were probed p70 S6K (Thr389), S6 ribosomal protein Ser235/236 [noted as S6 rp (Ser235/236)], eIF4G (Ser1108), RVFV nucleoprotein (NP), and β-actin as a loading control. (C) Data plotted represents fold change over mock from blots in (B). Mean values and standard deviations of the chemiluminescent signal intensity of three biological replicates are represented. All values were normalized to the loading control, β-actin, with concomitant background signal subtraction. Error bars represent the means ± SD; n=3.