Skip to main content
. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Antiviral Res. 2017 Apr 23;143:162–175. doi: 10.1016/j.antiviral.2017.04.011

Figure 5. p70 S6K pathway is activated following RVFV infection in vivo.

Figure 5

(A) BALB/c mice (groups of 3) were subcutaneously infected with RVFV ZH501 (1000 PFU/mouse). Control mice were sacrificed on day 0 and infected mice on days 3, 4, and 5. Liver and spleen were collected and placed in blue lysis buffer. Tissues were homogenized, spun, transferred to new tubes, heated at 100°C for 15 minutes, and then frozen at −80°C prior to RPPA analysis. Error bars represent standard error of the mean; n=3. (B) and (C) Immunohistochemistry for p70S 6K (Thr421) was performed as an additional determinant of activation of p70 S6K within the liver of infected mice. Panel B is an uninfected control and panel C is an infected day 4 sample. Arrows indicate an increase in p70 S6K staining.