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Chromatin immunoprecipitation (ChIP) with antibodies to histone H3 (positive control), p-cFos, and p-Smad2 in bone marrow mononuclear cells revealed the nuclear factor of activated T cells c1 (NFATc1) promoter associated with p-cFos in RANK ligand (RANKL)-treated cells.34 Treatment with RANKL plus activin A revealed both p-cFos and p-Smad2 associated with the NFATcl promoter.
