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. 2017 Jul 26;8:172. doi: 10.1186/s13287-017-0626-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Differentially expressed mRNAs and miRNAs detected by HTS and verified by real-time PCR. a Heat map of all mRNAs and miNRAs showing differences in the transcriptome of the colonies and control. Three independent samples of colonies and their preculture controls (pancreas) were harvested for HTS. b, c Verification of differentially expressed mRNAs and miRNAs by real-time PCR. *p < 0.05, **p < 0.01 and ***p < 0.005, comparison to the control group. CD133, CD44, ICAM1, DLL1 and CD38 were enriched in ring colonies. miR-21a-3p, miR-31-5p, miR-155-5p and miR-200c were upregulated while miR-217-5p, miR-802-5p, miR-375-5p and miR-216-5p were downregulated (Color figure online)