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Transmission electron micrographs of Caco-2 barriers after exposure to 50 nm SiO2-NPs. Caco-2 barriers cultured for 21 days were exposed for 9 h to 100 µg/mL 50 nm SiO2-NPs dispersed in (a,b) the absence of serum, and (c–f) the presence of 10% FBS. The arrows indicate some extracellular NPs and a few NPs observed inside the cells in different vesicles along the endolysosomal pathway. Abbreviations: E, endosome; L, lysosome; V, vesicle.
