Fig. 1.

Recombinant expression of LGI1. (A) Schematic representation of recombinant glycosylated LGI1 with signal peptide SP, His6, and T7 tags, leucine-rich region (LRR), and epitempin domain. (B) SDS/PAGE of purified LGI1: Coomassie blue-stained LGI1 (lane 1; 1 μg) and Western blot (0.1 μg) probed with anti-LGI1 (lane 2). (C) Western blots of 0.1 μg of purified LGI1 (lane 1) incubated in PNGase buffer (lanes 2 and 3) in the absence (lane 2) or presence of PNGase (lane 3) probed with anti-T7 antibody. To optimize protein resolution, the gel in C was run for a longer time than in B.