Fig. 7.

Formin or Cdc42 inhibition disrupts the formation of new adhesions, but not the maintenance of existing adhesions. (Left) Bright-field images showing MDCK cell aggregation at times before (0 h, 3 h) and after (5 h) addition of inhibitors at 3 h. (Scale bar, 50 µm.) (Right) Quantification of hanging-drop assay in the presence of the indicated inhibitors. Cells were binned into cluster classes: 1–10 cells, 11–20 cells, 21–50 cells, 51–100 cells, or >100 cells. The percentage of cells in each cluster size for each time point is indicated. The data shown are averaged from three independent experiments and errors bars represent SEM.