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Effects of inhibiting actin nucleating proteins and Rho GTPases in cells on collagen-I–coated 30-kPa and 60-kPa PA gels. Line graphs illustrate the effects on protrusive cell area by (A) SMIFH2, (B) CK666, (C) ML141, and (D) NSC23766. The solid line indicates the mean protrusive area for each condition and the error bars indicate SEM (n ≥ 10 cells per condition from at least three independent experiments).
