Fig. 4.

Breast cancer cells stably transfected with sh-FSIP1 display reduced growth when xenotransplanted in nude mice. SKBR3 cells stably transfected with sh-FSIP1 or sh-NC were injected into the flanks of BALB/c nu/nu mice and tumor volume measured by calipers for 27 d, after which the mice were killed. (A–C) Change (Δ) in tumor volume (A) and biweekly body weight (B) measurements, are shown together with Waterfall plot comparing Δ tumor volume in individual mice (C). (D) Ki67 staining of tissues. Mean ± SEM; n = 5 and 6 mice injected with sh-FSIP1 and sh-NC transfectants, respectively. Two-tailed Student's t test, *P ≤ 0.05, **P ≤ 0.01.