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. 2017 Jul 3;114(29):7707–7712. doi: 10.1073/pnas.1700286114

Fig. 2.

Fig. 2.

AmrZ inversely regulates H2- and H1-/H3-T6SSs. (A) AmrZ is a negative regulator for H2-T6SS. Level of LacZ activity of A2tl after overexpression of amrZ (pAmrZ) or in the presence of vector (pMMB) ± induction with IPTG. Graph represents mean ± SEM; n = 4; ANOVA Bonferroni posttest P < 0.001. (B) AmrZ represses H2-T6SS (tssB2), but activates H1-T6SS (tssA1) and H3-T6SS (vgrG3). qRT-PCR was performed on PA14rsmA overexpressing amrZ (pAmrZ) and compared with vector control (pMMB). Scatter plot of fold change gene expression with mean (n = 3). Statistical analysis was performed on the ΔΔCT values (ANOVA Bonferroni posttest P < 0.01 for all genes). (C) Fold gene repression of data shown in B.