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AmrZ binds the promoter regions of T6SS genes. Each reaction contains 5 nM 32P-labeled DNA and increasing concentrations of purified AmrZ, as indicated. Electrophoretic mobility shift assay was performed using DNA probes for (A) tssA1, tssA2, and (B) tssB3. In all cases, positive (adcA/algDl) or negative (algDs/algB) controls were used as previously published (18, 19). Asterisk indicates unspecific band.
