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Deletion of rsmA enables PldA secretion. Western blot analysis using anti-TEM to detect PldA-Bla expression shows expression in a PAO1rsmA mutant at 25 °C and secretion in a (A) H2-T6SS–dependent manner [tssB2 mutant (H2-)] and (B) VgrG4b-depenedent manner (vgrG4b mutant). Anti-RNA polymerase (RpoB) is used as a lysis control and anti-Hcp2 as a control for H2-T6SS activity.
