Fig. 7.

RsmA controls remote vgrG and T6SS effector genes. (A) qRT-PCR on a subset of vgrG genes (Left) and genes encoding known/putative effectors (Right) (SI Appendix, Fig. S1) in PA14 (WT) or a rsmA mutant. Scatter plot of fold change with mean (n = 3). Statistical analysis was performed on the ΔΔCT values (ANOVA Bonferroni posttest, P < 0.005 for all genes). (B) Western blot analysis using specific antibodies against selected VgrG proteins (VgrG2a, VgrG2b, and VgrG4b) and the T6SS effector PldB (PldB-Bla fusion detected with anti-TEM).