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. 2017 Jul 3;114(29):7707–7712. doi: 10.1073/pnas.1700286114

Fig. 9.

Fig. 9.

Coassembly of multiple T6SS machines. (A) Assembly of extended T6SS sheaths as seen by fluorescence microscopy. Fluorescent fusions of TssB1-Venus, TssB2-CFP, TssB3-CFP, or TssB3-sfGFP were expressed in PA14rsmA. The images shown are representative of >100 fields analyzed from at least four independent experiments. (B) Fluorescent fusion combinations of TssB1-Venus with TssB2-CFP (Upper), TssB1-Venus with TssB3-CFP (Middle), or TssB2-CFP with TssB3-sfGFP (Lower) were coexpressed in PA14rsmA. (Left) Bright field channel. The arrows point to the foci of interest in cells that have two different T6SS machines assembled. The images shown are representative of ≥100 fields analyzed from at least two independent experiments. (Scale bars, 1 μm.)