Fig. S6.

DLG1, Afadin, and phospho-ERM in uniaxially stretched cells. Immunostaining of endogenous E-cadherin and DLG1 (A), Afadin (B), or phospho-Ezrin (T567)/phosho-Radixin (T564)/phospho-Moesin (T558) (pERM) (C) in MDCK cells with and without 1 h application of 12% uniaxial stretch, showing the lack of polarization of DLG1, Afadin, and pERM upon uniaxial stretch. Similar results were obtained with longer duration of uniaxial stretch. Note that pERM stains the apical brush border and not cell–cell contacts. (Scale bars, 10 µm.)